Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine.
Identifieur interne : 000C35 ( Main/Exploration ); précédent : 000C34; suivant : 000C36Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine.
Auteurs : T V Chirkova [Russie] ; A N Naykhin ; G D Petukhova ; D A Korenkov ; S A Donina ; A N Mironov ; L G RudenkoSource :
- Clinical and vaccine immunology : CVI [ 1556-679X ] ; 2011.
Descripteurs français
- KwdFr :
- Cytokines (biosynthèse), Humains, Jeune adulte, Lymphocytes T CD4+ (immunologie), Lymphocytes T CD8+ (immunologie), Mémoire immunologique, Rappel de vaccin (), Sous-type H2N2 du virus de la grippe A (génétique), Sous-type H2N2 du virus de la grippe A (immunologie), Sous-type H5N2 du virus de la grippe A (génétique), Sous-type H5N2 du virus de la grippe A (immunologie), Vaccination (), Vaccins antigrippaux (administration et posologie), Vaccins antigrippaux (immunologie), Vaccins atténués (administration et posologie), Vaccins atténués (immunologie), Virus recombinants (immunologie).
- MESH :
- administration et posologie : Vaccins antigrippaux, Vaccins atténués.
- biosynthèse : Cytokines.
- génétique : Sous-type H2N2 du virus de la grippe A, Sous-type H5N2 du virus de la grippe A.
- immunologie : Lymphocytes T CD4+, Lymphocytes T CD8+, Sous-type H2N2 du virus de la grippe A, Sous-type H5N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués, Virus recombinants.
- Humains, Jeune adulte, Mémoire immunologique, Rappel de vaccin, Vaccination.
English descriptors
- KwdEn :
- CD4-Positive T-Lymphocytes (immunology), CD8-Positive T-Lymphocytes (immunology), Cytokines (biosynthesis), Humans, Immunization, Secondary (methods), Immunologic Memory, Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (immunology), Influenza A Virus, H5N2 Subtype (genetics), Influenza A Virus, H5N2 Subtype (immunology), Influenza Vaccines (administration & dosage), Influenza Vaccines (immunology), Reassortant Viruses (immunology), Vaccination (methods), Vaccines, Attenuated (administration & dosage), Vaccines, Attenuated (immunology), Young Adult.
- MESH :
- chemical , administration & dosage : Influenza Vaccines, Vaccines, Attenuated.
- chemical , biosynthesis : Cytokines.
- genetics : Influenza A Virus, H2N2 Subtype, Influenza A Virus, H5N2 Subtype.
- immunology : CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H5N2 Subtype, Influenza Vaccines, Reassortant Viruses, Vaccines, Attenuated.
- methods : Immunization, Secondary, Vaccination.
- Humans, Immunologic Memory, Young Adult.
Abstract
Cellular immune responses of both CD4 and CD8 memory/effector T cells were evaluated in healthy young adults who received two doses of live attenuated influenza A (H5N2) vaccine. The vaccine was developed by reassortment of nonpathogenic avian A/Duck/Potsdam/1402-6/68 (H5N2) and cold-adapted A/Leningrad/134/17/57 (H2N2) viruses. T-cell responses were measured by standard methods of intracellular cytokine staining of gamma interferon (IFN-γ)-producing cells and a novel T-cell recognition of antigen-presenting cells by protein capture (TRAP) assay based on the trogocytosis phenomenon, namely, plasma membrane exchange between interacting immune cells. TRAP enables the detection of activated trogocytosis-positive T cells after virus stimulation. We showed that two doses of live attenuated influenza A (H5N2) vaccine promoted both CD4 and CD8 T-memory-cell responses in peripheral blood of healthy young subjects in the clinical study. Significant differences in geometric mean titers (GMTs) of influenza A (H5N2)-specific IFN-γ(+) cells were observed at day 42 following the second vaccination, while peak levels of trogocytosis(+) T cells were detected earlier, on the 21st day after the second vaccination. The inverse correlation of baseline levels compared to postvaccine fold changes in GMTs of influenza-specific CD4 and CD8 T cells demonstrated that baseline levels of these specific cells could be considered a predictive factor of vaccine immunogenicity.
DOI: 10.1128/CVI.05116-11
PubMed: 21813657
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000166
- to stream PubMed, to step Curation: 000166
- to stream PubMed, to step Checkpoint: 000153
- to stream Ncbi, to step Merge: 000673
- to stream Ncbi, to step Curation: 000673
- to stream Ncbi, to step Checkpoint: 000673
- to stream Main, to step Merge: 000C39
- to stream Main, to step Curation: 000C35
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine.</title><author><name sortKey="Chirkova, T V" sort="Chirkova, T V" uniqKey="Chirkova T" first="T V" last="Chirkova">T V Chirkova</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Experimental Medicine, Acad. Pavlov St. 12, Saint Petersburg 197376, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Virology, Institute of Experimental Medicine, Acad. Pavlov St. 12, Saint Petersburg 197376</wicri:regionArea><wicri:noRegion>Saint Petersburg 197376</wicri:noRegion></affiliation></author><author><name sortKey="Naykhin, A N" sort="Naykhin, A N" uniqKey="Naykhin A" first="A N" last="Naykhin">A N Naykhin</name></author><author><name sortKey="Petukhova, G D" sort="Petukhova, G D" uniqKey="Petukhova G" first="G D" last="Petukhova">G D Petukhova</name></author><author><name sortKey="Korenkov, D A" sort="Korenkov, D A" uniqKey="Korenkov D" first="D A" last="Korenkov">D A Korenkov</name></author><author><name sortKey="Donina, S A" sort="Donina, S A" uniqKey="Donina S" first="S A" last="Donina">S A Donina</name></author><author><name sortKey="Mironov, A N" sort="Mironov, A N" uniqKey="Mironov A" first="A N" last="Mironov">A N Mironov</name></author><author><name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21813657</idno><idno type="pmid">21813657</idno><idno type="doi">10.1128/CVI.05116-11</idno><idno type="wicri:Area/PubMed/Corpus">000166</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000166</idno><idno type="wicri:Area/PubMed/Curation">000166</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000166</idno><idno type="wicri:Area/PubMed/Checkpoint">000153</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000153</idno><idno type="wicri:Area/Ncbi/Merge">000673</idno><idno type="wicri:Area/Ncbi/Curation">000673</idno><idno type="wicri:Area/Ncbi/Checkpoint">000673</idno><idno type="wicri:Area/Main/Merge">000C39</idno><idno type="wicri:Area/Main/Curation">000C35</idno><idno type="wicri:Area/Main/Exploration">000C35</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine.</title><author><name sortKey="Chirkova, T V" sort="Chirkova, T V" uniqKey="Chirkova T" first="T V" last="Chirkova">T V Chirkova</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Experimental Medicine, Acad. Pavlov St. 12, Saint Petersburg 197376, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Virology, Institute of Experimental Medicine, Acad. Pavlov St. 12, Saint Petersburg 197376</wicri:regionArea><wicri:noRegion>Saint Petersburg 197376</wicri:noRegion></affiliation></author><author><name sortKey="Naykhin, A N" sort="Naykhin, A N" uniqKey="Naykhin A" first="A N" last="Naykhin">A N Naykhin</name></author><author><name sortKey="Petukhova, G D" sort="Petukhova, G D" uniqKey="Petukhova G" first="G D" last="Petukhova">G D Petukhova</name></author><author><name sortKey="Korenkov, D A" sort="Korenkov, D A" uniqKey="Korenkov D" first="D A" last="Korenkov">D A Korenkov</name></author><author><name sortKey="Donina, S A" sort="Donina, S A" uniqKey="Donina S" first="S A" last="Donina">S A Donina</name></author><author><name sortKey="Mironov, A N" sort="Mironov, A N" uniqKey="Mironov A" first="A N" last="Mironov">A N Mironov</name></author><author><name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name></author></analytic><series><title level="j">Clinical and vaccine immunology : CVI</title><idno type="eISSN">1556-679X</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD4-Positive T-Lymphocytes (immunology)</term><term>CD8-Positive T-Lymphocytes (immunology)</term><term>Cytokines (biosynthesis)</term><term>Humans</term><term>Immunization, Secondary (methods)</term><term>Immunologic Memory</term><term>Influenza A Virus, H2N2 Subtype (genetics)</term><term>Influenza A Virus, H2N2 Subtype (immunology)</term><term>Influenza A Virus, H5N2 Subtype (genetics)</term><term>Influenza A Virus, H5N2 Subtype (immunology)</term><term>Influenza Vaccines (administration & dosage)</term><term>Influenza Vaccines (immunology)</term><term>Reassortant Viruses (immunology)</term><term>Vaccination (methods)</term><term>Vaccines, Attenuated (administration & dosage)</term><term>Vaccines, Attenuated (immunology)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cytokines (biosynthèse)</term><term>Humains</term><term>Jeune adulte</term><term>Lymphocytes T CD4+ (immunologie)</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Mémoire immunologique</term><term>Rappel de vaccin ()</term><term>Sous-type H2N2 du virus de la grippe A (génétique)</term><term>Sous-type H2N2 du virus de la grippe A (immunologie)</term><term>Sous-type H5N2 du virus de la grippe A (génétique)</term><term>Sous-type H5N2 du virus de la grippe A (immunologie)</term><term>Vaccination ()</term><term>Vaccins antigrippaux (administration et posologie)</term><term>Vaccins antigrippaux (immunologie)</term><term>Vaccins atténués (administration et posologie)</term><term>Vaccins atténués (immunologie)</term><term>Virus recombinants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Influenza Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A Virus, H5N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sous-type H2N2 du virus de la grippe A</term><term>Sous-type H5N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T CD4+</term><term>Lymphocytes T CD8+</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Sous-type H5N2 du virus de la grippe A</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term><term>CD8-Positive T-Lymphocytes</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A Virus, H5N2 Subtype</term><term>Influenza Vaccines</term><term>Reassortant Viruses</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization, Secondary</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Immunologic Memory</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Jeune adulte</term><term>Mémoire immunologique</term><term>Rappel de vaccin</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cellular immune responses of both CD4 and CD8 memory/effector T cells were evaluated in healthy young adults who received two doses of live attenuated influenza A (H5N2) vaccine. The vaccine was developed by reassortment of nonpathogenic avian A/Duck/Potsdam/1402-6/68 (H5N2) and cold-adapted A/Leningrad/134/17/57 (H2N2) viruses. T-cell responses were measured by standard methods of intracellular cytokine staining of gamma interferon (IFN-γ)-producing cells and a novel T-cell recognition of antigen-presenting cells by protein capture (TRAP) assay based on the trogocytosis phenomenon, namely, plasma membrane exchange between interacting immune cells. TRAP enables the detection of activated trogocytosis-positive T cells after virus stimulation. We showed that two doses of live attenuated influenza A (H5N2) vaccine promoted both CD4 and CD8 T-memory-cell responses in peripheral blood of healthy young subjects in the clinical study. Significant differences in geometric mean titers (GMTs) of influenza A (H5N2)-specific IFN-γ(+) cells were observed at day 42 following the second vaccination, while peak levels of trogocytosis(+) T cells were detected earlier, on the 21st day after the second vaccination. The inverse correlation of baseline levels compared to postvaccine fold changes in GMTs of influenza-specific CD4 and CD8 T cells demonstrated that baseline levels of these specific cells could be considered a predictive factor of vaccine immunogenicity.</div></front></TEI><affiliations><list><country><li>Russie</li></country></list><tree><noCountry><name sortKey="Donina, S A" sort="Donina, S A" uniqKey="Donina S" first="S A" last="Donina">S A Donina</name><name sortKey="Korenkov, D A" sort="Korenkov, D A" uniqKey="Korenkov D" first="D A" last="Korenkov">D A Korenkov</name><name sortKey="Mironov, A N" sort="Mironov, A N" uniqKey="Mironov A" first="A N" last="Mironov">A N Mironov</name><name sortKey="Naykhin, A N" sort="Naykhin, A N" uniqKey="Naykhin A" first="A N" last="Naykhin">A N Naykhin</name><name sortKey="Petukhova, G D" sort="Petukhova, G D" uniqKey="Petukhova G" first="G D" last="Petukhova">G D Petukhova</name><name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name></noCountry><country name="Russie"><noRegion><name sortKey="Chirkova, T V" sort="Chirkova, T V" uniqKey="Chirkova T" first="T V" last="Chirkova">T V Chirkova</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C35 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000C35 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21813657
|texte= Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21813657" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |